For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to RPL23A
Predicted to work with:
Mouse, Rat, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Plants, Zebrafish, Soybean, Xenopus tropicalis
Synthetic peptide, corresponding to a region within amino acids 106-156 of Human RPL23A according to NP_000975.2.
293T, HeLa and Jurkat whole cell lysates.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
Use at 2-10 µg/mg of lysate.
Use a concentration of 1 µg/ml.
This protein binds to a specific region on the 26S rRNA.
Belongs to the ribosomal protein L23P family.
N-terminus is methylated by METTL11A/NTM1.
Information by UniProt
60S ribosomal protein L23A antibody
Immunocytochemistry/ Immunofluorescence - Anti-RPL23A antibody (ab157110)
ICC/IF image of ab157110 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab157110 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (
ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunoprecipitation - Anti-RPL23A antibody (ab157110)
Detection of RPL23A by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: IP with an alternative Rabbit anti-EXOSC1 antibody.
Lane 2: IP with ab157110.
Lane 3: Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157110 at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
Western blot - Anti-RPL23A antibody (ab157110)
All lanes : Anti-RPL23A antibody (ab157110) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 18 kDa Exposure time: 3 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"