Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL23A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Plants, Zebrafish, Soybean, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 106-156 of Human RPL23A according to NP_000975.2.

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
IP Use at 2-10 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

Target

Images

  • ICC/IF image of ab157110 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab157110 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Detection of RPL23A by Western Blot of Immunprecipitate.

    Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:

    Lane 1: IP with an alternative Rabbit anti-EXOSC1 antibody.
    Lane 2: IP with ab157110.
    Lane 3: Control IgG.

    The subsequent Western blot of immunoprecipitates was done using ab157110 at 0.4 µg/ml.
    Detection: Chemiluminescence with exposure time of 30 seconds.
  • All lanes : Anti-RPL23A antibody (ab157110) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 18 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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