Key features and details
- Rabbit polyclonal to RPL23A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPL23A antibody
See all RPL23A primary antibodies
DescriptionRabbit polyclonal to RPL23A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Plants, Zebrafish, Soybean, Xenopus tropicalis
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 18 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThis protein binds to a specific region on the 26S rRNA.
Sequence similaritiesBelongs to the ribosomal protein L23P family.
modificationsN-terminus is methylated by METTL11A/NTM1.
- Information by UniProt
- 60S ribosomal protein L23A antibody
- FLJ27455 antibody
- L23A antibody
All lanes : Anti-RPL23A antibody (ab157110) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes
Detection of RPL23A by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: IP with an alternative Rabbit anti-EXOSC1 antibody.
Lane 2: IP with ab157110.
Lane 3: Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157110 at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab157110 has been referenced in 1 publication.
- Li F et al. Autophagic recycling plays a central role in maize nitrogen remobilization. Plant Cell 27:1389-408 (2015). WB . PubMed: 25944100