Product nameAnti-RPL23A antibody
See all RPL23A primary antibodies
DescriptionRabbit polyclonal to RPL23A
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Plants, Zebrafish, Soybean, Xenopus tropicalis
- 293T, HeLa and Jurkat whole cell lysate (ab7899). This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 18 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionThis protein binds to a specific region on the 26S rRNA.
Sequence similaritiesBelongs to the ribosomal protein L23P family.
modificationsN-terminus is methylated by METTL11A/NTM1.
- Information by UniProt
- 60S ribosomal protein L23A antibody
- FLJ27455 antibody
- L23A antibody
All lanes : Anti-RPL23A antibody (ab157110) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes
ICC/IF image of ab157110 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab157110 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Detection of RPL23A by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1: IP with an alternative Rabbit anti-EXOSC1 antibody.
Lane 2: IP with ab157110.
Lane 3: Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157110 at 0.4 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.