Anti-RPL23A antibody (ab223089)
Key features and details
- Rabbit polyclonal to RPL23A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL23A antibody
See all RPL23A primary antibodies -
Description
Rabbit polyclonal to RPL23A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human RPL23A aa 1-100.
Database link: P62750 -
Positive control
- WB: Rat brain lysate. IHC-P: Human testis tissue. ICC: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
This protein binds to a specific region on the 26S rRNA. -
Sequence similarities
Belongs to the ribosomal protein L23P family. -
Post-translational
modificationsN-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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Database links
- Entrez Gene: 507168 Cow
- Entrez Gene: 6147 Human
- Entrez Gene: 100043755 Mouse
- Entrez Gene: 268449 Mouse
- Entrez Gene: 360572 Rat
- GenBank: NP_000975.2 Human
- Omim: 602326 Human
- SwissProt: Q24JY1 Cow
see all -
Alternative names
- 60S ribosomal protein L23A antibody
- FLJ27455 antibody
- L23A antibody
see all
Images
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Anti-RPL23A antibody (ab223089) at 1/500 dilution + Rat brain lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Paraffin-embedded human testis tissue stained for RPL23A using ab223089 at 1/100 dilution in immunohistochemical analysis.
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A549 (human lung carcinoma cell line) cells stained for RPL23A (green) using ab223089 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223089 has not yet been referenced specifically in any publications.