Key features and details
- Rabbit polyclonal to RPL27A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPL27A antibody
See all RPL27A primary antibodies
DescriptionRabbit polyclonal to RPL27A
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Chimpanzee, Orangutan
- Human pancreas tissue, RT-4 and U-251 MG lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the ribosomal protein L15P family.
- Information by UniProt
- 60S ribosomal protein L27a antibody
- FLJ43464 antibody
- ribosomal protein L27a antibody
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling RPL27A in the cytoplasm of exocrine glandular cells using ab204979 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling RPL27A in the cytosol and endoplasmic reticulum using ab204979 at 2 µg/ml.
All lanes : Anti-RPL27A antibody (ab204979) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
ab204979 has not yet been referenced specifically in any publications.