Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL27A
  • Host species

    Rabbit
  • Specificity

    ab74731 detects endogenous levels of total RPL27A protein.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    synthesized peptide derived from C-terminal region of human RPL27A.

  • Positive control

    • extracts from COLO cells and A549 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 17 kDa.
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

Target

Images

  • All lanes : Anti-RPL27A antibody (ab74731) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 17 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescence analysis of A549 cells, using ab74731 at 1/500 dilution with (+) or without (-) immunising peptide.

References

This product has been referenced in:

  • Modelska A  et al. The malignant phenotype in breast cancer is driven by eIF4A1-mediated changes in the translational landscape. Cell Death Dis 6:e1603 (2015). WB ; Human . Read more (PubMed: 25611378) »
  • Chandra S  et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci 126:3782-95 (2013). Read more (PubMed: 23750012) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Mouse Cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Permeabilization
Yes - 0.5% Triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 07 2013

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (gradient)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 26 2013

Abreviews
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (gradient)
Sample
Human Cell lysate - whole cell (U2OS cells)
Specification
U2OS cells
Treatment
Time course of serum stimulation
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 13 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (normal and tumor colon cells)
Loading amount
30 µg
Specification
normal and tumor colon cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 12 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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