Western blot abreview for Anti-RPL27A antibody

Good
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (gradient)
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification
Mouse embryonic fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: DAKO
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
West Femto
Bands
Specific: 15 kDa Non-specific: 130 kDa
Exposure
15 minute(s) and 0 second(s)

Additional data

Additional Notes
1-3. Mouse embryonic fibroblasts

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Submitted Jun 26 2013

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