Overview

  • Product name

    Anti-RPL27/RPL27A antibody
    See all RPL27/RPL27A primary antibodies
  • Description

    Rabbit polyclonal to RPL27/RPL27A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Cynomolgus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human RPL27/RPL27A aa 2-136.
    Sequence:

    GKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPR KVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVF RDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF


    Database link: P61353

  • Positive control

    • WB: EC109 and HEK-293T whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human colon cancer tissue.
  • General notes

     This product was previously labelled as RPL27

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 16 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

Images

  • Paraffin-embedded human colon cancer tissue stained for RPL27/RPL27A using ab236906 at 1/50 dilution in immunohistochemical analysis.

  • All lanes : Anti-RPL27/RPL27A antibody (ab236906) at 1/500 dilution

    Lane 1 : EC109 whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/15000 dilution

    Predicted band size: 16 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RPL27/RPL27A using ab236906 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab236906 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236906.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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