Anti-RPL27/RPL27A antibody (ab236906)
Key features and details
- Rabbit polyclonal to RPL27/RPL27A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL27/RPL27A antibody
See all RPL27/RPL27A primary antibodies -
Description
Rabbit polyclonal to RPL27/RPL27A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Cynomolgus monkey
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Immunogen
Recombinant full length protein corresponding to Human RPL27/RPL27A aa 2-136.
Sequence:GKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPR KVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVF RDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF
Database link: P61353 -
Positive control
- WB: EC109 and HEK-293T whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human colon cancer tissue.
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General notes
This product was previously labelled as RPL27
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab236906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Relevance
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27 is a ribosomal protein that is a component of the 60S subunit. It belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
Database links
- Entrez Gene: 396280 Chicken
- Entrez Gene: 404137 Cow
- Entrez Gene: 403688 Dog
- Entrez Gene: 6155 Human
- Entrez Gene: 19942 Mouse
- Entrez Gene: 100037995 Pig
- Entrez Gene: 64306 Rat
- Entrez Gene: 325618 Zebrafish
see all -
Alternative names
- 60S ribosomal protein L27 antibody
- Ribosomal protein L27 antibody
- RPL 27 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL27/RPL27A antibody (ab236906)Paraffin-embedded human colon cancer tissue stained for RPL27/RPL27A using ab236906 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-RPL27/RPL27A antibody (ab236906)All lanes : Anti-RPL27/RPL27A antibody (ab236906) at 1/500 dilution
Lane 1 : EC109 whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 16 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RPL27/RPL27A antibody (ab236906)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RPL27/RPL27A using ab236906 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab236906 has not yet been referenced specifically in any publications.
