Anti-RPL27/RPL27A antibody (ab236906)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RPL27/RPL27A antibody
See all RPL27/RPL27A primary antibodies -
Description
Rabbit polyclonal to RPL27/RPL27A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish, Cynomolgus monkey
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Immunogen
Recombinant full length protein corresponding to Human RPL27/RPL27A aa 2-136.
Sequence:GKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPR KVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVF RDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF
Database link: P61353 -
Positive control
- WB: EC109 and HEK-293T whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human colon cancer tissue.
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General notes
This product was previously labelled as RPL27
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Relevance
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27 is a ribosomal protein that is a component of the 60S subunit. It belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
Database links
- Entrez Gene: 396280 Chicken
- Entrez Gene: 404137 Cow
- Entrez Gene: 403688 Dog
- Entrez Gene: 6155 Human
- Entrez Gene: 19942 Mouse
- Entrez Gene: 100037995 Pig
- Entrez Gene: 64306 Rat
- Entrez Gene: 325618 Zebrafish
see all -
Alternative names
- 60S ribosomal protein L27 antibody
- Ribosomal protein L27 antibody
- RPL 27 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL27/RPL27A antibody (ab236906)Paraffin-embedded human colon cancer tissue stained for RPL27/RPL27A using ab236906 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-RPL27/RPL27A antibody (ab236906)All lanes : Anti-RPL27/RPL27A antibody (ab236906) at 1/500 dilution
Lane 1 : EC109 whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 16 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RPL27/RPL27A antibody (ab236906)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RPL27/RPL27A using ab236906 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
References
ab236906 has not yet been referenced specifically in any publications.
