Product nameAnti-RPL27/RPL27A antibody
See all RPL27/RPL27A primary antibodies
DescriptionRabbit polyclonal to RPL27/RPL27A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Zebrafish
Synthetic peptide within Human RPL27/RPL27A aa 86-136. The exact sequence is proprietary.
Database link: P61353
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: RPL27/RPL27A IP in HEK-293T whole cell lysate.
This product was previously labelled as RPL27
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to RPL27/RPL27A immobilized on solid support.
Our Abpromise guarantee covers the use of ab241409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27 is a ribosomal protein that is a component of the 60S subunit. It belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
- 60S ribosomal protein L27 antibody
- Ribosomal protein L27 antibody
- RPL 27 antibody
RPL27/RPL27A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241409 at 6 µg per reaction. Western blot was performed on the immunoprecipitate usign ab241409 at 1 µg/mL.
Lane 1: ab241409 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-239T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-RPL27/RPL27A antibody (ab241409) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
Prepared using NETN lysis buffer.
ab241409 has not yet been referenced specifically in any publications.