Anti-RPL27/RPL27A antibody (ab241417)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RPL27/RPL27A antibody
See all RPL27/RPL27A primary antibodies -
Description
Rabbit polyclonal to RPL27/RPL27A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Zebrafish
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Immunogen
Synthetic peptide within Human RPL27/RPL27A aa 86-136. The exact sequence is proprietary.
Database link: P61353 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as RPL27
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to RPL27/RPL27A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/25000. Predicted molecular weight: 16 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27 is a ribosomal protein that is a component of the 60S subunit. It belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
Database links
- Entrez Gene: 396280 Chicken
- Entrez Gene: 404137 Cow
- Entrez Gene: 403688 Dog
- Entrez Gene: 6155 Human
- Entrez Gene: 19942 Mouse
- Entrez Gene: 100037995 Pig
- Entrez Gene: 64306 Rat
- Entrez Gene: 325618 Zebrafish
see all -
Alternative names
- 60S ribosomal protein L27 antibody
- Ribosomal protein L27 antibody
- RPL 27 antibody
Images
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Western blot - Anti-RPL27/RPL27A antibody (ab241417)All lanes : Anti-RPL27/RPL27A antibody (ab241417) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 10 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-RPL27/RPL27A antibody (ab241417)RPL27/RPL27A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigena) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241417 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241417 at 1 µg/ml.
Lane 1: ab241417 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared in NETN buffer.
Datasheets and documents
References
ab241417 has not yet been referenced specifically in any publications.
