Anti-RPL27/RPL27A antibody (ab241417)
Key features and details
- Rabbit polyclonal to RPL27/RPL27A
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL27/RPL27A antibody
See all RPL27/RPL27A primary antibodies -
Description
Rabbit polyclonal to RPL27/RPL27A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Pig, Zebrafish
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Immunogen
Synthetic peptide within Human RPL27/RPL27A aa 86-136. The exact sequence is proprietary.
Database link: P61353 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as RPL27
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to RPL27/RPL27A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/25000. Predicted molecular weight: 16 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27 is a ribosomal protein that is a component of the 60S subunit. It belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. -
Database links
- Entrez Gene: 396280 Chicken
- Entrez Gene: 404137 Cow
- Entrez Gene: 403688 Dog
- Entrez Gene: 6155 Human
- Entrez Gene: 19942 Mouse
- Entrez Gene: 100037995 Pig
- Entrez Gene: 64306 Rat
- Entrez Gene: 325618 Zebrafish
see all -
Alternative names
- 60S ribosomal protein L27 antibody
- Ribosomal protein L27 antibody
- RPL 27 antibody
Images
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Western blot - Anti-RPL27/RPL27A antibody (ab241417)All lanes : Anti-RPL27/RPL27A antibody (ab241417) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
Exposure time: 10 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-RPL27/RPL27A antibody (ab241417)RPL27/RPL27A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigena) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241417 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241417 at 1 µg/ml.
Lane 1: ab241417 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared in NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241417 has not yet been referenced specifically in any publications.
