Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to internal sequence amino acids of Human RPL28.

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138125 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 16 kDa.

Target

Images

  • Anti-RPL28 antibody (ab138125) at 1/500 dilution + HeLa cell lysate at 30 µg

    Predicted band size: 16 kDa

References

This product has been referenced in:

  • An H & Harper JW Systematic analysis of ribophagy in human cells reveals bystander flux during selective autophagy. Nat Cell Biol 20:135-143 (2018). Read more (PubMed: 29230017) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Colon)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Colon
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 26 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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