Overview

  • Product name

  • Description

    Mouse polyclonal to RPL29
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-159 of Human RPL29

  • Positive control

    • Human pancreas tissue lysate and 293T cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 21 and 25 kDa (predicted molecular weight: 18 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities

      Belongs to the ribosomal protein L29e family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 60S ribosomal protein L29 antibody
      • Cell surface heparin binding protein HIP antibody
      • Cell surface heparin-binding protein HIP antibody
      • Heparin/heparan sulfate interacting protein antibody
      • Heparin/heparan sulfate-binding protein antibody
      • HIP antibody
      • HP/HS interacting protein antibody
      • HUMRPL29 antibody
      • MGC88589 antibody
      • OTTHUMP00000212470 antibody
      • OTTHUMP00000212473 antibody
      • OTTHUMP00000212474 antibody
      • OTTHUMP00000212475 antibody
      • OTTHUMP00000212476 antibody
      • OTTHUMP00000212477 antibody
      • Ribosomal protein L29 antibody
      • ribosomal protein YL43 homologue antibody
      • RL29_HUMAN antibody
      • rpl29 antibody
      • RPL29_3_370 antibody
      • RPL29P10 antibody
      see all

    Images

    • Anti-RPL29 antibody (ab67196) at 1/500 dilution + human pancreas tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 18 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-RPL29 antibody (ab67196) at 1/500 dilution

      Lane 1 : RPL29 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 18 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?

    References

    ab67196 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you very much for contacting us with your question.

    Ab67196 was raised against the full-length humanprotein from amino acids 1-159, and since it is polyclonal it will recognize several different regions along the length of the protein. So, it will recognize both the N and C termini.

    I hope that this information will be useful, but if you have any further questions please let me know and I'll be happy to help.

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