Anti-RPL3 antibody (ab154882)

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Product name

Anti-RPL3 antibody
See all RPL3 primary antibodies
Description

Rabbit polyclonal to RPL3
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
Immunogen

Recombinant fragment corresponding to a region within amino acids 1-171 of Human RPL3 (UniProt ID: P39023).
Positive control
- A549 cells; Human AdCA xenograft tissue

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab154882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P		1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/1000.

Function

The L3 protein is a component of the large subunit of cytoplasmic ribosomes.
Sequence similarities

Belongs to the ribosomal protein L3P family.
Cellular localization

Cytoplasm.
Target information above from: UniProt accession P39023 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 418016 Chicken
- Entrez Gene: 282688 Cow
- Entrez Gene: 6122 Human
- Entrez Gene: 380033 Xenopus laevis
- Entrez Gene: 100145083 Xenopus tropicalis
- Entrez Gene: 322571 Zebrafish
- Omim: 604163 Human
- SwissProt: P39872 Cow
- SwissProt: P39023 Human
- Unigene: 119598 Human
see all
Alternative names
- 60S ribosomal protein L3 antibody
- HIV-1 TAR RNA-binding protein B antibody
- HIV1 TAR RNA binding protein B antibody
- MGC103388 antibody
- MGC104284 antibody
- MGC72934 antibody
- Ribosomal protein L3 antibody
- Ribosomal protein L3, isoform a antibody
- RL3_HUMAN antibody
- rpl3 antibody
- TARBP B antibody
- TARBP-B antibody
see all

Western blot - Anti-RPL3 antibody (ab154882)

Anti-RPL3 antibody (ab154882) at 1/1000 dilution + A549 whole cell lysate at 30 µg

Predicted band size: 46 kDa

10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)

Immunofluorescence analysis of methanol fixed A549 cells, labeling RPL3 using ab154882 at 1/500 dilution. Lower image costained with Hoerchst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)

Immunohistochemical analysis of paraffin embedded Human AdCA xenograft tissue, labeling RPL3 using ab154882 at 1/100 dilution.

ab154882 has not yet been referenced specifically in any publications.

Publishing research using ab154882? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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