Anti-RPL3 antibody (ab154882)
Overview
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Product name
Anti-RPL3 antibody
See all RPL3 primary antibodies -
Description
Rabbit polyclonal to RPL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 1-171 of Human RPL3 (UniProt ID: P39023).
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Positive control
- A549 cells; Human AdCA xenograft tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 46 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
The L3 protein is a component of the large subunit of cytoplasmic ribosomes. -
Sequence similarities
Belongs to the ribosomal protein L3P family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 418016 Chicken
- Entrez Gene: 282688 Cow
- Entrez Gene: 6122 Human
- Entrez Gene: 380033 Xenopus laevis
- Entrez Gene: 100145083 Xenopus tropicalis
- Entrez Gene: 322571 Zebrafish
- Omim: 604163 Human
- SwissProt: P39872 Cow
see all -
Alternative names
- 60S ribosomal protein L3 antibody
- HIV-1 TAR RNA-binding protein B antibody
- HIV1 TAR RNA binding protein B antibody
see all
Images
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Western blot - Anti-RPL3 antibody (ab154882)Anti-RPL3 antibody (ab154882) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)Immunofluorescence analysis of methanol fixed A549 cells, labeling RPL3 using ab154882 at 1/500 dilution. Lower image costained with Hoerchst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)Immunohistochemical analysis of paraffin embedded Human AdCA xenograft tissue, labeling RPL3 using ab154882 at 1/100 dilution.
Protocols
References
ab154882 has been referenced in 1 publication.
- Nemmara VV et al. The Development of Benzimidazole-Based Clickable Probes for the Efficient Labeling of Cellular Protein Arginine Deiminases (PADs). ACS Chem Biol 13:712-722 (2018). PubMed: 29341591
