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    rpl3-antibody-ab154882.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-RPL3 antibody (ab154882)

  • Datasheet
  • SDS
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Western blot - Anti-RPL3 antibody (ab154882)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)

Key features and details

  • Rabbit polyclonal to RPL3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RPL3 antibody
    See all RPL3 primary antibodies
  • Description

    Rabbit polyclonal to RPL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-171 of Human RPL3 (UniProt ID: P39023).

  • Positive control

    • A549 cells; Human AdCA xenograft tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    The L3 protein is a component of the large subunit of cytoplasmic ribosomes.
  • Sequence similarities

    Belongs to the ribosomal protein L3P family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P39023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 418016 Chicken
    • Entrez Gene: 282688 Cow
    • Entrez Gene: 6122 Human
    • Entrez Gene: 380033 Xenopus laevis
    • Entrez Gene: 100145083 Xenopus tropicalis
    • Entrez Gene: 322571 Zebrafish
    • Omim: 604163 Human
    • SwissProt: P39872 Cow
    • SwissProt: P39023 Human
    • Unigene: 119598 Human
    see all
  • Alternative names

    • 60S ribosomal protein L3 antibody
    • HIV-1 TAR RNA-binding protein B antibody
    • HIV1 TAR RNA binding protein B antibody
    • MGC103388 antibody
    • MGC104284 antibody
    • MGC72934 antibody
    • Ribosomal protein L3 antibody
    • Ribosomal protein L3, isoform a antibody
    • RL3_HUMAN antibody
    • rpl3 antibody
    • TARBP B antibody
    • TARBP-B antibody
    see all

Images

  • Western blot - Anti-RPL3 antibody (ab154882)
    Western blot - Anti-RPL3 antibody (ab154882)
    Anti-RPL3 antibody (ab154882) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)
    Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)
    Immunofluorescence analysis of methanol fixed A549 cells, labeling RPL3 using ab154882 at 1/500 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)
    Immunohistochemical analysis of paraffin embedded Human AdCA xenograft tissue, labeling RPL3 using ab154882 at 1/100 dilution.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154882? Please let us know so that we can cite the reference in this datasheet.

ab154882 has been referenced in 1 publication.

  • Nemmara VV  et al. The Development of Benzimidazole-Based Clickable Probes for the Efficient Labeling of Cellular Protein Arginine Deiminases (PADs). ACS Chem Biol 13:712-722 (2018). PubMed: 29341591

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