Anti-RPL3 antibody (ab154882)

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Western blot - Anti-RPL3 antibody (ab154882)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)

Key features and details

  • Rabbit polyclonal to RPL3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RPL3 antibody
    See all RPL3 primary antibodies

  • Description

    Rabbit polyclonal to RPL3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis

  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-171 of Human RPL3 (UniProt ID: P39023).

  • Positive control

    • A549 cells; Human AdCA xenograft tissue

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

Images

  • Western blot - Anti-RPL3 antibody (ab154882)
    Western blot - Anti-RPL3 antibody (ab154882)
    Anti-RPL3 antibody (ab154882) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)
    Immunocytochemistry/ Immunofluorescence - Anti-RPL3 antibody (ab154882)
    Immunofluorescence analysis of methanol fixed A549 cells, labeling RPL3 using ab154882 at 1/500 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL3 antibody (ab154882)
    Immunohistochemical analysis of paraffin embedded Human AdCA xenograft tissue, labeling RPL3 using ab154882 at 1/100 dilution.

References (1)

Publishing research using ab154882? Please let us know so that we can cite the reference in this datasheet.

ab154882 has been referenced in 1 publication.

  • Nemmara VV  et al. The Development of Benzimidazole-Based Clickable Probes for the Efficient Labeling of Cellular Protein Arginine Deiminases (PADs). ACS Chem Biol 13:712-722 (2018). PubMed: 29341591

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