Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human RPL3 (internal sequence). The exact sequence is proprietary.
    Database link: P39023

  • Positive control

    • WB: Jurkat and Raji whole cell lysates. IHC-P: Mouse intestine and brain tissue; 4T1 xenograft tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-RPL3 antibody (ab228638) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 46 kDa



    10% SDS-PAGE

  • Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for RPL3 (green) using ab228638 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342.

  • Paraffin-embedded mouse intestine tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded 4T1 xenograft tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.

References

ab228638 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228638.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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