Product nameAnti-RPL3 antibody
See all RPL3 primary antibodies
DescriptionRabbit polyclonal to RPL3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
Recombinant fragment within Human RPL3 (internal sequence). The exact sequence is proprietary.
Database link: P39023
- WB: Jurkat and Raji whole cell lysates. IHC-P: Mouse intestine and brain tissue; 4T1 xenograft tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionThe L3 protein is a component of the large subunit of cytoplasmic ribosomes.
Sequence similaritiesBelongs to the ribosomal protein L3P family.
- Information by UniProt
- 60S ribosomal protein L3 antibody
- HIV-1 TAR RNA-binding protein B antibody
- HIV1 TAR RNA binding protein B antibody
All lanes : Anti-RPL3 antibody (ab228638) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 46 kDa
Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for RPL3 (green) using ab228638 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342.
Paraffin-embedded mouse intestine tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded 4T1 xenograft tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse brain tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
ab228638 has not yet been referenced specifically in any publications.