Key features and details
- Rabbit polyclonal to RPL3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPL3 antibody
See all RPL3 primary antibodies
DescriptionRabbit polyclonal to RPL3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey
Recombinant fragment within Human RPL3 (internal sequence). The exact sequence is proprietary.
Database link: P39023
- WB: Jurkat and Raji whole cell lysates. IHC-P: Mouse intestine and brain tissue; 4T1 xenograft tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionThe L3 protein is a component of the large subunit of cytoplasmic ribosomes.
Sequence similaritiesBelongs to the ribosomal protein L3P family.
- Information by UniProt
- 60S ribosomal protein L3 antibody
- HIV-1 TAR RNA-binding protein B antibody
- HIV1 TAR RNA binding protein B antibody
All lanes : Anti-RPL3 antibody (ab228638) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 46 kDa
Methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for RPL3 (green) using ab228638 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342.
Paraffin-embedded mouse intestine tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded 4T1 xenograft tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse brain tissue stained for RPL3 with ab228638 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228638 has not yet been referenced specifically in any publications.