
Recombinant Anti-RPL30 antibody [EPR11624] (ab170930)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RPL30 antibody [EPR11624]
See all RPL30 primary antibodies -
Description
Rabbit monoclonal [EPR11624] to RPL30 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human RPL30. The exact sequence is proprietary.
Database link: P62888 -
Positive control
- A549, HepG2, Jurkat and PC-3 cell lysates, Human gastric carcinoma & pancreas tissues, HepG2 cells, immunoprecipitation pellet from Jurkat whole cell lysate (ab7899)
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/10000 - 1/50000. Predicted molecular weight: 13 kDa. | |
ICC/IF | 1/100 - 1/250. | |
IP | 1/10 - 1/100. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the ribosomal protein L30e family. - Information by UniProt
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Database links
- Entrez Gene: 6156 Human
- Entrez Gene: 19946 Mouse
- Entrez Gene: 100362027 Rat
- Entrez Gene: 64640 Rat
- Omim: 180467 Human
- SwissProt: P62888 Human
- SwissProt: P62889 Mouse
- SwissProt: P62890 Rat
see all -
Alternative names
- 60S ribosomal protein L30 antibody
- L30 antibody
- Ribosomal protein L30 antibody
see all
Images
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Immunofluorescence analysis of HepG2 cells labeling RPL30 with ab170930 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RPL30 with ab170930 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling RPL30 with ab170930 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab170930 at 1/10 dilution.
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All lanes : Anti-RPL30 antibody [EPR11624] (ab170930) at 1/10000 dilution
Lane 1 : A549 cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : PC-3 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 13 kDa
Protocols
Datasheets and documents
References
ab170930 has not yet been referenced specifically in any publications.