Recombinant
RabMAb

Recombinant Anti-RPL30 antibody [EPR11624] (ab170930)

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Overview

  • Product name

    Anti-RPL30 antibody [EPR11624]
    See all RPL30 primary antibodies

  • Description

    Rabbit monoclonal [EPR11624] to RPL30

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human RPL30. The exact sequence is proprietary.
    Database link: P62888

  • Positive control

    • A549, HepG2, Jurkat and PC-3 cell lysates, Human gastric carcinoma & pancreas tissues, HepG2 cells, immunoprecipitation pellet from Jurkat whole cell lysate (ab7899)

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11624

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 13 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-RPL30 antibody [EPR11624] (ab170930)
      Immunocytochemistry/ Immunofluorescence - Anti-RPL30 antibody [EPR11624] (ab170930)

      Immunofluorescence analysis of HepG2 cells labeling RPL30 with ab170930 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RPL30 with ab170930 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)

      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling RPL30 with ab170930 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-RPL30 antibody [EPR11624] (ab170930)
      Immunoprecipitation - Anti-RPL30 antibody [EPR11624] (ab170930)

      Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab170930 at 1/10 dilution.

    • Western blot - Anti-RPL30 antibody [EPR11624] (ab170930)
      Western blot - Anti-RPL30 antibody [EPR11624] (ab170930)
      All lanes : Anti-RPL30 antibody [EPR11624] (ab170930) at 1/10000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : HepG2 cell lysates
      Lane 3 : Jurkat cell lysates
      Lane 4 : PC-3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 13 kDa

    References

    ab170930 has not yet been referenced specifically in any publications.

    Publishing research using ab170930? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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