Name: Anti-RPL30 antibody [EPR11624]
Brand: RabMAb
SKU: ab170930

Product name

Anti-RPL30 antibody [EPR11624]
See all RPL30 primary antibodies
Description

Rabbit monoclonal [EPR11624] to RPL30
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Human RPL30. The exact sequence is proprietary.
Database link: P62888
Positive control
- A549, HepG2, Jurkat and PC-3 cell lysates, Human gastric carcinoma & pancreas tissues, HepG2 cells, immunoprecipitation pellet from Jurkat whole cell lysate (ab7899)
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11624
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab170930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Predicted molecular weight: 13 kDa.
ICC/IF		1/100 - 1/250.
IP		1/10 - 1/100.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt.

Sequence similarities

Belongs to the ribosomal protein L30e family.
Target information above from: UniProt accession P62888 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6156 Human
- Entrez Gene: 19946 Mouse
- Entrez Gene: 100362027 Rat
- Entrez Gene: 64640 Rat
- Omim: 180467 Human
- SwissProt: P62888 Human
- SwissProt: P62889 Mouse
- SwissProt: P62890 Rat
- Unigene: 400295 Human
- Unigene: 358632 Mouse
- Unigene: 439801 Mouse
- Unigene: 161950 Rat
- Unigene: 3359 Rat
- Unigene: 36878 Rat
see all
Alternative names
- 60S ribosomal protein L30 antibody
- L30 antibody
- Ribosomal protein L30 antibody
- RL30_HUMAN antibody
- RPL30 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-RPL30 antibody [EPR11624] (ab170930)

Immunofluorescence analysis of HepG2 cells labeling RPL30 with ab170930 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RPL30 with ab170930 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL30 antibody [EPR11624] (ab170930)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling RPL30 with ab170930 at 1/100 dilution.
Immunoprecipitation - Anti-RPL30 antibody [EPR11624] (ab170930)

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab170930 at 1/10 dilution.
Western blot - Anti-RPL30 antibody [EPR11624] (ab170930)

All lanes : Anti-RPL30 antibody [EPR11624] (ab170930) at 1/10000 dilution

Lane 1 : A549 cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : PC-3 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 13 kDa

ab170930 has not yet been referenced specifically in any publications.

Publishing research using ab170930? Please let us know so that we can cite the reference in this datasheet.

Anti-RPL30 antibody [EPR11624] (ab170930)

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