Recombinant Anti-RPL30 antibody [EPR11624] - BSA and Azide free (ab249601)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11624] to RPL30 - BSA and Azide free
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RPL30 antibody [EPR11624] - BSA and Azide free
See all RPL30 primary antibodies -
Description
Rabbit monoclonal [EPR11624] to RPL30 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WB, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human RPL30. The exact sequence is proprietary.
Database link: P62888 -
General notes
ab249601 is the carrier-free version of ab170930.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the ribosomal protein L30e family. - Information by UniProt
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Database links
- Entrez Gene: 6156 Human
- Entrez Gene: 19946 Mouse
- Entrez Gene: 100362027 Rat
- Entrez Gene: 64640 Rat
- Omim: 180467 Human
- SwissProt: P62888 Human
- SwissProt: P62889 Mouse
- SwissProt: P62890 Rat
see all -
Alternative names
- 60S ribosomal protein L30 antibody
- L30 antibody
- Ribosomal protein L30 antibody
see all
Images
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This data was developed using ab170930, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RPL30 with ab170930 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab170930, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling RPL30 with ab170930 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RPL30 antibody [EPR11624] (ab170930) at 1/10000 dilution
Lane 1 : A549 cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : PC-3 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 13 kDaThis data was developed using ab170930, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249601 has not yet been referenced specifically in any publications.