Overview

  • Product name

    Anti-RPL32 antibody
  • Description

    Rabbit polyclonal to RPL32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human RPL32 aa 1-135.
    Sequence:

    MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFK GQILMPNIGYGSNKKTKHMLPSGFRKFLVHNVKELEVLLMCNKSYCAEIA HNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE


    Database link: P62910

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human lung cancer and colon cancer tissue. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-RPL32 antibody (ab229758) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 16 kDa



    15% SDS-PAGE gel.

  • Paraffin-embedded human lung cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human colon cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RPL32 (green) using ab229758 at 1/1000 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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