Key features and details
- Rabbit polyclonal to RPL32
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPL32 antibody
DescriptionRabbit polyclonal to RPL32
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human lung cancer and colon cancer tissue. ICC/IF: MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 16 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
Sequence similaritiesBelongs to the ribosomal protein L32e family.
- Information by UniProt
- 60S ribosomal protein L32 antibody
- L32 antibody
- PP9932 antibody
All lanes : Anti-RPL32 antibody (ab229758) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
15% SDS-PAGE gel.
Paraffin-embedded human lung cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RPL32 (green) using ab229758 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229758 has not yet been referenced specifically in any publications.