Product nameAnti-RPL32 antibody
DescriptionRabbit polyclonal to RPL32
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Human lung cancer and colon cancer tissue. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 16 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
Sequence similaritiesBelongs to the ribosomal protein L32e family.
- Information by UniProt
- 60S ribosomal protein L32 antibody
- L32 antibody
- PP9932 antibody
All lanes : Anti-RPL32 antibody (ab229758) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 16 kDa
15% SDS-PAGE gel.
Paraffin-embedded human lung cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human colon cancer tissue stained for RPL32 with ab229758 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for RPL32 (green) using ab229758 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
ab229758 has not yet been referenced specifically in any publications.