Overview

  • Product name

    Anti-RPL39 antibody
  • Description

    Rabbit polyclonal to RPL39
  • Host species

    Rabbit
  • Specificity

    ab74740 detects endogenous levels of total RPL39 protein.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human RPL39.

  • Positive control

    • WB: HepG2 and COLO cell extracts ICC/IF: A549 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab74740 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 7 kDa.
ELISA 1/60000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance

    RPL39 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the S39E family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein is the smallest, and one of the most basic, proteins of the ribosome. This gene is cotranscribed with the U69 small nucleolar RNA gene, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • Database links

  • Alternative names

    • 2810465O16Rik antibody
    • 60S ribosomal protein L39 antibody
    • OTTHUMP00000023908 antibody
    • OTTMUSP00000018582 antibody
    • Ribosomal protein L39 antibody
    • RP23-43O20.1 antibody
    see all

Images

  • All lanes : Anti-RPL39 antibody (ab74740) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : COLO cell extracts
    Lane 3 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 7 kDa
    Observed band size: 7 kDa

  • ab74740 at 1/500 dilution staining RPL39 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

This product has been referenced in:

  • Dave B  et al. Targeting RPL39 and MLF2 reduces tumor initiation and metastasis in breast cancer by inhibiting nitric oxide synthase signaling. Proc Natl Acad Sci U S A 111:8838-43 (2014). Human . Read more (PubMed: 24876273) »
See 1 Publication for this product

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