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    rpl4-antibody-ab154907.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-RPL4 antibody (ab154907)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Anti-RPL4 antibody (ab154907)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)
  • Immunocytochemistry - Anti-RPL4 antibody (ab154907)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)

Key features and details

  • Rabbit polyclonal to RPL4
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RPL4 antibody
    See all RPL4 primary antibodies
  • Description

    Rabbit polyclonal to RPL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 60-340 of Human RPL4 (Uniprot ID: P36578).

  • Positive control

    • WB: Raji, K562 and NCI-H929 whole cell lysates; IHC-P: Human U87 xenograft, mouse colon and pancreas tissue; IF: HepG2 whole cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/1000.
Notes
ICC
Use at an assay dependent concentration.
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P
1/100 - 1/1000.

Target

  • Sequence similarities

    Belongs to the ribosomal protein L4P family.
  • Target information above from: UniProt accession P36578 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 415551 Chicken
    • Entrez Gene: 6124 Human
    • Entrez Gene: 67891 Mouse
    • Entrez Gene: 64302 Rat
    • Omim: 180479 Human
    • SwissProt: Q58DW0 Cow
    • SwissProt: P36578 Human
    • SwissProt: Q9D8E6 Mouse
    • SwissProt: P50878 Rat
    • Unigene: 186350 Human
    • Unigene: 644628 Human
    • Unigene: 280083 Mouse
    • Unigene: 1133 Rat
    see all
  • Alternative names

    • 60S ribosomal protein L1 antibody
    • 60S ribosomal protein L4 antibody
    • L4 antibody
    • Ribosomal protein L4 antibody
    • RL4_HUMAN antibody
    • rpl4 antibody
    see all

Images

  • Western blot - Anti-RPL4 antibody (ab154907)
    Western blot - Anti-RPL4 antibody (ab154907)
    All lanes : Anti-RPL4 antibody (ab154907) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 48 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)

    Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RPL4 with ab154907 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry - Anti-RPL4 antibody (ab154907)
    Immunocytochemistry - Anti-RPL4 antibody (ab154907)

    Immunofluorescence analysis of  ice-cold MeOH HepG2 (human liver hepatocellular carcinoma cell line) whole cell labeling RPL4 at cytoplasm with ab154907 at 1/500 dilution. Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154907? Please let us know so that we can cite the reference in this datasheet.

ab154907 has been referenced in 1 publication.

  • Ouyang H  et al. Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Front Physiol 8:281 (2017). WB . PubMed: 28533755

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