Anti-RPL4 antibody (ab154907)
Key features and details
- Rabbit polyclonal to RPL4
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL4 antibody
See all RPL4 primary antibodies -
Description
Rabbit polyclonal to RPL4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 60-340 of Human RPL4 (Uniprot ID: P36578).
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Positive control
- WB: Raji, K562 and NCI-H929 whole cell lysates; IHC-P: Human U87 xenograft, mouse colon and pancreas tissue; IF: HepG2 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/100 - 1/1000.
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| Notes |
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ICC
Use at an assay dependent concentration. |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
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IHC-P
1/100 - 1/1000. |
Target
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Sequence similarities
Belongs to the ribosomal protein L4P family. - Information by UniProt
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Database links
- Entrez Gene: 415551 Chicken
- Entrez Gene: 6124 Human
- Entrez Gene: 67891 Mouse
- Entrez Gene: 64302 Rat
- Omim: 180479 Human
- SwissProt: Q58DW0 Cow
- SwissProt: P36578 Human
- SwissProt: Q9D8E6 Mouse
see all -
Alternative names
- 60S ribosomal protein L1 antibody
- 60S ribosomal protein L4 antibody
- L4 antibody
see all
Images
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Western blot - Anti-RPL4 antibody (ab154907)All lanes : Anti-RPL4 antibody (ab154907) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 48 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RPL4 with ab154907 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry - Anti-RPL4 antibody (ab154907)Immunofluorescence analysis of ice-cold MeOH HepG2 (human liver hepatocellular carcinoma cell line) whole cell labeling RPL4 at cytoplasm with ab154907 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154907 has been referenced in 1 publication.
- Ouyang H et al. Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Front Physiol 8:281 (2017). WB . PubMed: 28533755