Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 142-297 of Human RPL5.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji cell lysates; Mouse brain; HepG2 cells; Gastric CA N87 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab137617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
  • Involvement in disease

    Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities

    Belongs to the ribosomal protein L18P family.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60S ribosomal protein L5 antibody
    • DBA6 antibody
    • L5 antibody
    • MSTP030 antibody
    • PPP1R135 antibody
    • protein phosphatase 1, regulatory subunit 135 antibody
    • Ribosomal protein L5 antibody
    • RL5 antibody
    • RL5_HUMAN antibody
    • RP L5 antibody
    • RPL 5 antibody
    • RPL5 antibody
    • U21 RNA antibody
    • U21RNA antibody
    see all

Images

  • All lanes : Anti-RPL5 antibody (ab137617) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLaS3 whole cell lysate
    Lane 4 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 34 kDa



    12% SDS PAGE
  • Anti-RPL5 antibody (ab137617) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 34 kDa



    10% SDS PAGE
  • ab137617 at 1/500 dilution staining RPL5 in 4% paraformaldehyde (room temperature for 15 min) fixed HepG2 cells by Immunofluorescence (green). Blue staining: Hoechst 33343.
  • ab137617 at 1/100 dilution, staining RPL5 in paraffin embedded Gastric CA N87 xenograft tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Timpano S & Uniacke J Human Cells Cultured under Physiological Oxygen Utilize Two Cap-binding Proteins to recruit Distinct mRNAs for Translation. J Biol Chem 291:10772-82 (2016). Read more (PubMed: 27002144) »
See 1 Publication for this product

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