Key features and details
- Rabbit polyclonal to RPL5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPL5 antibody
See all RPL5 primary antibodies
DescriptionRabbit polyclonal to RPL5
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig
Recombinant fragment, corresponding to a region within amino acids 142-297 of Human RPL5.
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji cell lysates; Mouse brain; HepG2 cells; Gastric CA N87 xenograft tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 34 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
Involvement in diseaseDefects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similaritiesBelongs to the ribosomal protein L18P family.
Cellular localizationCytoplasm. Nucleus > nucleolus.
- Information by UniProt
- 60S ribosomal protein L5 antibody
- DBA6 antibody
- L5 antibody
All lanes : Anti-RPL5 antibody (ab137617) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLaS3 whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS PAGE
Anti-RPL5 antibody (ab137617) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 34 kDa
10% SDS PAGE
ab137617 at 1/500 dilution staining RPL5 in 4% paraformaldehyde (room temperature for 15 min) fixed HepG2 cells by Immunofluorescence (green). Blue staining: Hoechst 33343.
ab137617 at 1/100 dilution, staining RPL5 in paraffin embedded Gastric CA N87 xenograft tissue by Immunohistochemistry.
ab137617 has been referenced in 1 publication.
- Timpano S & Uniacke J Human Cells Cultured under Physiological Oxygen Utilize Two Cap-binding Proteins to recruit Distinct mRNAs for Translation. J Biol Chem 291:10772-82 (2016). PubMed: 27002144