Key features and details
- Rabbit polyclonal to RPL5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPL5 antibody
See all RPL5 primary antibodies
DescriptionRabbit polyclonal to RPL5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
- 293T; HeLa; Jurkat and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 34 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
Involvement in diseaseDefects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similaritiesBelongs to the ribosomal protein L18P family.
Cellular localizationCytoplasm. Nucleus > nucleolus.
- Information by UniProt
- 60S ribosomal protein L5 antibody
- DBA6 antibody
- L5 antibody
All lanes : Anti-RPL5 antibody (ab157099) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Exposure time: 10 seconds
Detection of RPL5 by Western Blot of Immunprecipitate.
Lane 1: ab157099 at 1µg/ml labelling RPL5 in 293T whole cell lysate immunoprecipitated using ab157099 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
ab157099 has been referenced in 1 publication.
- Turi Z et al. Perturbation of RNA Polymerase I transcription machinery by ablation of HEATR1 triggers the RPL5/RPL11-MDM2-p53 ribosome biogenesis stress checkpoint pathway in human cells. Cell Cycle 17:92-101 (2018). PubMed: 29143558