Overview

  • Product name

  • Description

    Rabbit polyclonal to RPL5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human RPL5 (N terminal).
    Database link: P46777

  • Positive control

    • SW620 and Raji cell extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab186857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 34 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
  • Involvement in disease

    Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6) [MIM:612561]. DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities

    Belongs to the ribosomal protein L18P family.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60S ribosomal protein L5 antibody
    • DBA6 antibody
    • L5 antibody
    • MSTP030 antibody
    • PPP1R135 antibody
    • protein phosphatase 1, regulatory subunit 135 antibody
    • Ribosomal protein L5 antibody
    • RL5 antibody
    • RL5_HUMAN antibody
    • RP L5 antibody
    • RPL 5 antibody
    • RPL5 antibody
    • U21 RNA antibody
    • U21RNA antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186857. Blue DAPI for nuclear staining.
  • All lanes : Anti-RPL5 antibody (ab186857) at 1/500 dilution

    Lane 1 : SW620 cell extracts
    Lane 2 : Raji cell extracts

    Predicted band size: 34 kDa

References

ab186857 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186857.
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