Anti-RPL7 antibody (ab72550)
Key features and details
- Rabbit polyclonal to RPL7
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL7 antibody -
Description
Rabbit polyclonal to RPL7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Catfish, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to a region between residue 200 and the C-terminus (residue 248) of human RPL7 (NP_000962.2)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
Use a concentration of 1 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
Use a concentration of 1 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Binds to G-rich structures in 28S rRNA and in mRNAs. Plays a regulatory role in the translation apparatus; inhibits cell-free translation of mRNAs. -
Sequence similarities
Belongs to the ribosomal protein L30P family. - Information by UniProt
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Database links
- Entrez Gene: 6129 Human
- Entrez Gene: 19989 Mouse
- Entrez Gene: 297755 Rat
- Omim: 604166 Human
- SwissProt: P18124 Human
- SwissProt: P14148 Mouse
- SwissProt: P05426 Rat
- Unigene: 421257 Human
see all -
Alternative names
- 60S ribosomal protein L7 antibody
- humL7 1 antibody
- L7 antibody
see all
Images
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All lanes : Anti-RPL7 antibody (ab72550)
Lane 1 : U2OS whole cell lysate treated with control siRNA
Lane 2 : U2OS whole cell lysate treated with RPL7 siRNA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/1 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocked with 2% milk for 1 hour at 18°C.
Incubated with the primary antibody in 2% milk for 18 hours at 4°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma and bronchioalveolar carcinoma pleural effusion tissue labelling RPL7 with ab72550 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-RPL7 antibody (ab72550) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Detection of Human RPL7 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72550 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
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IHC image of ab72550 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab72550 has been referenced in 23 publications.
- Zinnall U et al. HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Nat Commun 13:2727 (2022). PubMed: 35585045
- Tian G et al. Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins. EMBO J 40:e106183 (2021). PubMed: 34010456
- Kraushar ML et al. Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit. Mol Cell 81:304-322.e16 (2021). PubMed: 33357414
- Lin Y et al. eIF3 Associates with 80S Ribosomes to Promote Translation Elongation, Mitochondrial Homeostasis, and Muscle Health. Mol Cell 79:575-587.e7 (2020). PubMed: 32589965
- Aarum J et al. Enzymatic degradation of RNA causes widespread protein aggregation in cell and tissue lysates. EMBO Rep 21:e49585 (2020). PubMed: 32945072