Overview

  • Product name
  • Description
    Rabbit polyclonal to RPL7A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 15-296 of Human RPL7A (UniProt P62424).

  • Positive control
    • A431, Jurkat, Raji, Neuro2A, GL261 and C8D30 whole cell lysates; A431 cells; Human lung adenocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-RPL7A antibody (ab155147) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS PAGE
  • All lanes : Anti-RPL7A antibody (ab155147) at 1/3000 dilution

    Lane 1 : Neuro2A whole cell lysate
    Lane 2 : GL261 whole cell lysate
    Lane 3 : C8D30 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RPL7A with ab155147 at 1/500 dilution.
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling RPL7A with ab155147 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab155147 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Tobacco Recombinant protein (recombinant protein from Nicotiana tabacum produce)
Gel Running Conditions
Reduced Denaturing (12 %)
Loading amount
10 µg
Specification
recombinant protein from Nicotiana tabacum produce
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Claire Rosnoblet

Verified customer

Submitted Jul 17 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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