Western blot abreview for Anti-RPL7A antibody

Excellent
Abreviews
Application
Western blot
Sample
Tobacco Recombinant protein (recombinant protein from Nicotiana tabacum produce)
Gel Running Conditions
Reduced Denaturing (12 %)
Loading amount
10 µg
Specification
recombinant protein from Nicotiana tabacum produce
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS Tween 0.1 % BSA 2 %
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Lumiglo
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 33 kDa

Additional data

Abcam response
Thank you for participating in our AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in tobacco, a species that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in tobacco which will help us assess how well the antibody works in this species.

Mrs. Claire Rosnoblet

Verified customer

Submitted Jul 17 2018

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