Product nameAnti-RPL7A antibody
See all RPL7A primary antibodies
DescriptionRabbit polyclonal to RPL7A
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residue 215 and the C-terminus (residue 266) of human RPL7A (NP_000963.1).
- Whole cell lysate from HeLa, MCF7 or 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).|
Involvement in diseaseNote=Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene.
Sequence similaritiesBelongs to the ribosomal protein L7Ae family.
- Information by UniProt
- 60S ribosomal protein L7a antibody
- L7A antibody
- MGC103146 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling RPL7A with ab70753 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-RPL7A antibody (ab70753) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : HeLa whole cell lysate at 4 µg
Lane 3 : MCF7 whole cell lysate at 20 µg
Lane 4 : 293T whole cell lysate at 20 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
This product has been referenced in:
- Sahoo MR et al. Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC. EMBO Rep 18:241-263 (2017). Read more (PubMed: 28039207) »
- Yang PY et al. Activity-based proteome profiling of potential cellular targets of Orlistat--an FDA-approved drug with anti-tumor activities. J Am Chem Soc 132:656-66 (2010). Read more (PubMed: 20028024) »