Recombinant Anti-RPL7A antibody [EPR12310] (ab172478)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12310] to RPL7A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RPL7A antibody [EPR12310]
See all RPL7A primary antibodies -
Description
Rabbit monoclonal [EPR12310] to RPL7A -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment within Human RPL7A aa 1-200. The exact sequence is proprietary.
Database link: P62424 -
Positive control
- HeLa, MCF7, 293T, Jurkat, Raw 264.7 and NIH/3T3 cell lysate; Human brain, hepatocellular carcinoma and uterus tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12310 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
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IHC-P |
Human
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WB |
Human
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All applications |
Rat
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Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Involvement in disease
Note=Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene. -
Sequence similarities
Belongs to the ribosomal protein L7Ae family. - Information by UniProt
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Database links
- Entrez Gene: 6130 Human
- Entrez Gene: 27176 Mouse
- Entrez Gene: 296596 Rat
- Omim: 185640 Human
- SwissProt: P62424 Human
- SwissProt: P12970 Mouse
- SwissProt: P62425 Rat
- Unigene: 499839 Human
see all -
Alternative names
- 60S ribosomal protein L7a antibody
- L7A antibody
- MGC103146 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling RPL7A with ab172478 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling RPL7A with ab172478 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RPL7A with ab172478 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RPL7A antibody [EPR12310] (ab172478) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa
Protocols
Datasheets and documents
References (1)
ab172478 has been referenced in 1 publication.
- Knight JR et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res 41:4185-97 (2013). PubMed: 23462953