Anti-RPL7A antibody [EPR12310] (ab172478)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-RPL7A antibody [EPR12310]
See all RPL7A primary antibodies -
DescriptionRabbit monoclonal [EPR12310] to RPL7A
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human RPL7A. The exact sequence is proprietary.
Database link: P62424 -
Positive control
- HeLa, MCF7, 293T, Jurkat, Raw 264.7 and NIH/3T3 cell lysate; Human brain, hepatocellular carcinoma and uterus tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR12310
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- NIH 3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- Jurkat whole cell lysate (ab7899)
- 293T whole cell lysate (ab95494)
Applications
Our Abpromise guarantee covers the use of ab172478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 30 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Involvement in diseaseNote=Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene.
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Sequence similaritiesBelongs to the ribosomal protein L7Ae family.
- Information by UniProt
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Database links
- Entrez Gene: 6130 Human
- Entrez Gene: 27176 Mouse
- Entrez Gene: 296596 Rat
- Omim: 185640 Human
- SwissProt: P62424 Human
- SwissProt: P12970 Mouse
- SwissProt: P62425 Rat
- Unigene: 499839 Human
see all -
Alternative names
- 60S ribosomal protein L7a antibody
- L7A antibody
- MGC103146 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.com/ps/products/172/ab172478/Images/ab172478-194765-ab1724784.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling RPL7A with ab172478 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.com/ps/products/172/ab172478/Images/ab172478-194764-ab1724783.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling RPL7A with ab172478 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.com/ps/products/172/ab172478/Images/ab172478-194763-ab1724782.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RPL7A with ab172478 at 1/50 dilution.
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![Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)](https://www.abcam.com/ps/products/172/ab172478/Images/ab172478-194762-ab1724781.jpg)
Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)All lanes : Anti-RPL7A antibody [EPR12310] (ab172478) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa
Datasheets and documents
References
ab172478 has not yet been referenced specifically in any publications.
