RecombinantRabMAb

Recombinant Anti-RPL7A antibody [EPR12310] (ab172478)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Anti-RPL7A antibody [EPR12310] (ab172478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12310] to RPL7A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RPL7A antibody [EPR12310]
    See all RPL7A primary antibodies

  • Description

    Rabbit monoclonal [EPR12310] to RPL7A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human RPL7A aa 1-200. The exact sequence is proprietary.
    Database link: P62424

  • Positive control

    • HeLa, MCF7, 293T, Jurkat, Raw 264.7 and NIH/3T3 cell lysate; Human brain, hepatocellular carcinoma and uterus tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12310

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)

      Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling RPL7A with ab172478 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)

      Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling RPL7A with ab172478 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)

      Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RPL7A with ab172478 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)
      Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)
      All lanes : Anti-RPL7A antibody [EPR12310] (ab172478) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : Raw 264.7 cell lysate
      Lane 6 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 30 kDa

    • Anti-RPL7A antibody [EPR12310] (ab172478)
      Anti-RPL7A antibody [EPR12310] (ab172478)

    References (1)

    Publishing research using ab172478? Please let us know so that we can cite the reference in this datasheet.

    ab172478 has been referenced in 1 publication.

    • Knight JR  et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res 41:4185-97 (2013). PubMed: 23462953

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