RecombinantRabMAb

Recombinant Anti-RPL7A antibody [EPR12310] (ab172478)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)
  • Anti-RPL7A antibody [EPR12310] (ab172478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12310] to RPL7A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Carrier Free

Overview

  • Product name

    Anti-RPL7A antibody [EPR12310]
    See all RPL7A primary antibodies

  • Description

    Rabbit monoclonal [EPR12310] to RPL7A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant fragment within Human RPL7A aa 1-200. The exact sequence is proprietary.
    Database link: P62424

  • Positive control

    • HeLa, MCF7, 293T, Jurkat, Raw 264.7 and NIH/3T3 cell lysate; Human brain, hepatocellular carcinoma and uterus tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12310

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)

    Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling RPL7A with ab172478 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)

    Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling RPL7A with ab172478 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody [EPR12310] (ab172478)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RPL7A with ab172478 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)
    Western blot - Anti-RPL7A antibody [EPR12310] (ab172478)
    All lanes : Anti-RPL7A antibody [EPR12310] (ab172478) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Raw 264.7 cell lysate
    Lane 6 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 30 kDa

  • Anti-RPL7A antibody [EPR12310] (ab172478)
    Anti-RPL7A antibody [EPR12310] (ab172478)

References (1)

Publishing research using ab172478? Please let us know so that we can cite the reference in this datasheet.

ab172478 has been referenced in 1 publication.

  • Knight JR  et al. Active regulator of SIRT1 is required for ribosome biogenesis and function. Nucleic Acids Res 41:4185-97 (2013). PubMed: 23462953

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