Overview

  • Product name

  • Description

    Goat polyclonal to RPL8
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis, Zebrafish, Japanese ricefish, Orangutan
  • Immunogen

    Synthetic peptide:

    KAGRAYHKYKAKRNC

    , corresponding to internal sequence amino acids 181-195 of Human RPL8

  • Positive control

    • HepG2 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab63941 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • ab63941 (2.5 µg/ml) staining RPL8 in paraffin-embedded human spleen following steamed antigen retrieval with citrate buffer pH 6.

    • Anti-RPL8 antibody (ab63941) at 0.01 µg/ml + HepG2 cell lysate at 35 µg

      Developed using the ECL technique.

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • ab63941 (2.5 µg/ml) staining RPL8 in paraffin-embedded human cerebral cortex following steamed antigen retrieval with citrate buffer pH 6.

    References

    ab63941 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab63941.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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