Overview

  • Product name
    Anti-RPL8 antibody [EPR11630]
    See all RPL8 primary antibodies
  • Description
    Rabbit monoclonal [EPR11630] to RPL8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human RPL8.

  • Positive control
    • HepG2, Jurkat, SKBR-3 and Molt4 cell lysates. HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11630
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    Images

    • All lanes : Anti-RPL8 antibody [EPR11630] (ab169538) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : Molt4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • Immunoprecipitation. ab169538 at 1/10000 staining RPL8 in Jurkat cell lysate immunoprecipitated using ab169538 at 1/10. Predicted band size : 28 kDa.
    • Immunofluorescent analysis of HepG2cells labeling RPL8 with ab169538 at 1/250.

    References

    This product has been referenced in:
    • Juszkiewicz S & Hegde RS Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination. Mol Cell 65:743-750.e4 (2017). Read more (PubMed: 28065601) »

    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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