Anti-RPL9 antibody (ab50384)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-RPL9 antibody
See all RPL9 primary antibodies -
DescriptionRabbit polyclonal to RPL9
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ELISAmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Drosophila melanogaster, Zebrafish -
Immunogen
A region within synthetic peptide: LILEGNDIEL VSNSAALIQQ ATTVKNKDIR KFLDGIYVSE KGTVQQADE, corresponding to C terminal amino acids 144-192 of Human RPL9
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Positive control
- Jurkat cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading...
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PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab50384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
ELISA | 1/312500. Titre using peptide based assay 1/312500. |
Target
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Sequence similaritiesBelongs to the ribosomal protein L6P family.
- Information by UniProt
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Database links
- Entrez Gene: 282884 Cow
- Entrez Gene: 34526 Drosophila melanogaster
- Entrez Gene: 6133 Human
- Entrez Gene: 20005 Mouse
- Entrez Gene: 100360449 Rat
- Entrez Gene: 100362838 Rat
- Entrez Gene: 100364388 Rat
- Entrez Gene: 100364457 Rat
see all -
Alternative names
- 60S ribosomal protein L9 antibody
- DKFZp313J1510 antibody
- FLJ27456 antibody
see all
Images
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Lane 1 : Molecular weight markers
Lane 2 : Anti-RPL9 antibody (ab50384) at 1.25 µg/ml
Lane 2 : Jurkat cell lysate at 10 µg
Secondary
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
Protocols
Datasheets and documents
References
This product has been referenced in:
- Miettinen TP & Björklund M Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions. Nucleic Acids Res 43:1019-34 (2015). Read more (PubMed: 25550424) »