Key features and details
- Rabbit monoclonal [EP13752] to RPL9
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RPL9 antibody [EP13752]
See all RPL9 primary antibodies
DescriptionRabbit monoclonal [EP13752] to RPL9
Tested applicationsSuitable for: IP, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human RPL9 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P32969
- HepG2, HeLa, HTC-116 and Jurkat whole cell lysate (ab7899); Human brain tissue; HTC-116 and HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab182556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/20 - 1/40.|
|WB||1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the ribosomal protein L6P family.
- Information by UniProt
- 60S ribosomal protein L9 antibody
- DKFZp313J1510 antibody
- FLJ27456 antibody
All lanes : Anti-RPL9 antibody [EP13752] (ab182556) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HCT-116 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RPL9 with ab182556 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling RPL9 with ab182556 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling RPL9 with ab182556 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Western blot analysis of HepG2 cell lysate immunoprecipitated with ab182556 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab182556 has been referenced in 2 publications.
- Vinayak J et al. Human La binds mRNAs through contacts to the poly(A) tail. Nucleic Acids Res 46:4228-4240 (2018). PubMed: 29447394
- Baik IH et al. Knockdown of RPL9 expression inhibits colorectal carcinoma growth via the inactivation of Id-1/NF-?B signaling axis. Int J Oncol 49:1953-1962 (2016). PubMed: 27633352