Anti-RPLP0 antibody (ab88872)
Key features and details
- Mouse polyclonal to RPLP0
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPLP0 antibody
See all RPLP0 primary antibodies -
Description
Mouse polyclonal to RPLP0 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RPLP0. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Ribosomal protein P0 is the functional equivalent of E.coli protein L10. -
Sequence similarities
Belongs to the ribosomal protein L10P family. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 6175 Human
- Omim: 180510 Human
- SwissProt: P05388 Human
- Unigene: 546285 Human
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Alternative names
- 36B4 antibody
- 60S acidic ribosomal protein P0 antibody
- 60S ribosomal protein L10E antibody
see all
Images
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Anti-RPLP0 antibody (ab88872) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 34 kDa
Observed band size: 34 kDa -
All lanes : Anti-RPLP0 antibody (ab88872) at 1 µg/ml
Lane 1 : RPLP0 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa -
ab88872 at 10µg/ml staining RPLP0 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab88872 has been referenced in 5 publications.
- Yoshikawa H et al. Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC. Elife 7:N/A (2018). PubMed: 30095066
- Deblois G et al. ERRa mediates metabolic adaptations driving lapatinib resistance in breast cancer. Nat Commun 7:12156 (2016). WB ; Human . PubMed: 27402251
- Taha MS et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). ICC/IF ; Human . PubMed: 24658146
- Hong EJ et al. Loss of estrogen-related receptor a promotes hepatocarcinogenesis development via metabolic and inflammatory disturbances. Proc Natl Acad Sci U S A 110:17975-80 (2013). WB ; Mouse . PubMed: 24127579
- Cheshenko N et al. HSV usurps eukaryotic initiation factor 3 subunit M for viral protein translation: novel prevention target. PLoS One 5:e11829 (2010). WB ; Human . PubMed: 20676407