Overview

  • Product name

    Anti-RPLP0 antibody [EP15646]
    See all RPLP0 primary antibodies
  • Description

    Rabbit monoclonal [EP15646] to RPLP0
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human RPLP0 aa 1-200. The exact sequence is proprietary.
    Database link: P05388

  • Positive control

    • 293, HeLa, Jurkat, A375, C6, RAW264.7, PC12 and NIH 3T3 cell lysates; mouse kidney, mouse spleen, rat brain and rat spleen lysates; Human stomach tissue, mouse liver tissue, rat cerebral cortex tissue; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP15646
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
  • Sequence similarities

    Belongs to the ribosomal protein L10P family.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • 36B4 antibody
    • 60S acidic ribosomal protein P0 antibody
    • 60S ribosomal protein L10E antibody
    • Acidic ribosomal phosphoprotein P0 antibody
    • Arbp antibody
    • L10E antibody
    • LP0 antibody
    • MGC107165 antibody
    • MGC107166 antibody
    • MGC111226 antibody
    • MGC88175 antibody
    • OTTMUSP00000015585 antibody
    • P0 antibody
    • PRLP0 antibody
    • Ribosomal phosphoprotein, acidic, P0 antibody
    • Ribosomal protein P0 antibody
    • Ribosomal protein, large, P0 antibody
    • RLA0_HUMAN antibody
    • rplP0 antibody
    • RPP0 antibody
    see all

Images

  • All lanes : Anti-RPLP0 antibody [EP15646] (ab192866) at 1/20000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A375 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • All lanes : Anti-RPLP0 antibody [EP15646] (ab192866) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW264.7 cell lysate
    Lane 7 : PC12 cell lysate
    Lane 8 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded rmouse liver tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

References

This product has been referenced in:

  • Matthes F  et al. Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis. Cell Death Discov 4:4 (2018). Read more (PubMed: 29531801) »
  • Conotte S  et al. Metabonomic profiling of chronic intermittent hypoxia in a mouse model. Respir Physiol Neurobiol N/A:N/A (2018). WB . Read more (PubMed: 29522877) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 13 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver sample, Muscle sample, RAW and HeLa cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
50 µg
Specification
Liver sample, Muscle sample, RAW and HeLa cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 18 2016

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