Overview

  • Product name

    Anti-RPLP0 antibody [EP15646] - BSA and Azide free
    See all RPLP0 primary antibodies
  • Description

    Rabbit monoclonal [EP15646] to RPLP0 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human RPLP0 aa 1-200. The exact sequence is proprietary.
    Database link: P05388

  • Positive control

    • IHC-P: Human stomach tissue.
  • General notes

    Ab236120 is the carrier-free version of ab192866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP15646
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
  • Sequence similarities

    Belongs to the ribosomal protein L10P family.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • 36B4 antibody
    • 60S acidic ribosomal protein P0 antibody
    • 60S ribosomal protein L10E antibody
    • Acidic ribosomal phosphoprotein P0 antibody
    • Arbp antibody
    • L10E antibody
    • LP0 antibody
    • MGC107165 antibody
    • MGC107166 antibody
    • MGC111226 antibody
    • MGC88175 antibody
    • OTTMUSP00000015585 antibody
    • P0 antibody
    • PRLP0 antibody
    • Ribosomal phosphoprotein, acidic, P0 antibody
    • Ribosomal protein P0 antibody
    • Ribosomal protein, large, P0 antibody
    • RLA0_HUMAN antibody
    • rplP0 antibody
    • RPP0 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded rmouse liver tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192866).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192866).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling RPLP0 with ab192866 at a 1/1000 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192866).

  • Immunohistochemical analysis of paraffin embedded Human stomach tissue sections labeling RPLP0 using ab192866 at a 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192866).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab236120 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236120.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up