Key features and details
- Rabbit polyclonal to RPLP2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPLP2 antibody
See all RPLP2 primary antibodies
DescriptionRabbit polyclonal to RPLP2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Monkey
Recombinant fragment, corresponding to a region within amino acids 1-99 of Human RPLP2 (P05387).
- 293T, A431, H1299, HeLa, HepG2, Molt4, Raji whole cell lysates; Human colon carcinoma tissue; A431 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 78% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 12 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionPlays an important role in the elongation step of protein synthesis.
Sequence similaritiesBelongs to the ribosomal protein L12P family.
- Information by UniProt
- 2700049I22Rik antibody
- 60S acidic ribosomal protein P2 antibody
- Acidic ribosomal phosphoprotein P2 antibody
All lanes : Anti-RPLP2 antibody (ab154958) at 1/1000 dilution
Lane 1 : Molt4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa
15% SDS PAGE
Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling RPLP2 with ab154958 at 1/500.
Immunofluorescence analysis of methanol fixed A431 cells labeling RPLP2 with ab154958 at 1/500. Lower panel shows costaining with Hoechst 33342.
ab154958 has been referenced in 1 publication.
- Campos RK et al. RPLP1 and RPLP2 Are Essential Flavivirus Host Factors That Promote Early Viral Protein Accumulation. J Virol 91:N/A (2017). PubMed: 27974556