Key features and details
- Rabbit polyclonal to RPN2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPN2 antibody
See all RPN2 primary antibodies
DescriptionRabbit polyclonal to RPN2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
- WB: HeLa, 293T or Jurkat whole cell lysate. IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 69 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEssential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Tissue specificityExpressed in all tissues tested.
Sequence similaritiesBelongs to the SWP1 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 63 kDa subunit precursor antibody
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit antibody
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 antibody
Detection of Human RPN2 by Western Blot of Immunoprecipitates. 0.5 or 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab186117 used for IP at 6 µg. For blotting immunoprecipitated RPN2, ab186117 was used at 1 µg/ml.
All lanes : Anti-RPN2 antibody (ab186117) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
Exposure time: 3 seconds
ab186117 has not yet been referenced specifically in any publications.