Overview

  • Product name

  • Description

    Rabbit polyclonal to RPN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide within Human RPN2 aa 350-400. The exact sequence is proprietary.
    Sequence:

    VEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYP A


    Database link: P04844

  • Positive control

    • WB: HeLa, 293T or Jurkat whole cell lysate. IP: HeLa cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • Detection of Human RPN2 by Western Blot of Immunoprecipitates. 0.5 or 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab186117 used for IP at 6 µg. For blotting immunoprecipitated RPN2, ab186117 was used at 1 µg/ml.

  • All lanes : Anti-RPN2 antibody (ab186117) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 69 kDa


    Exposure time: 3 seconds

References

ab186117 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186117.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up