Key features and details
- Rabbit polyclonal to RPN2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPN2 antibody
See all RPN2 primary antibodies
DescriptionRabbit polyclonal to RPN2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Orangutan
- WB: RT4 and U-251 MG sp cell lysates. IHC-P: Human epididymis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa.|
FunctionEssential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Tissue specificityExpressed in all tissues tested.
Sequence similaritiesBelongs to the SWP1 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 63 kDa subunit precursor antibody
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit antibody
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 antibody
All lanes : Anti-RPN2 antibody (ab224347) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 69 kDa
Paraffin-embedded human epididymis tissue stained for RPN2 using ab224347 at 1/50 in immunohistochemical analysis.
ab224347 has not yet been referenced specifically in any publications.