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Rabbit polyclonal to RPP25
antigen sequence: ASLSVLKNVP GLAILLSKDA LDPRQPGYQP PNPHPGPSSP PAAPASKRSL GEPAAGEGSA KRSQPEP, corresponding to Human RPP25.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
||1/500 - 1/1000. Predicted molecular weight: 21 kDa.
Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA.
Belongs to the histone-like Alba family.
Information by UniProt
- FLJ20374 antibody
- Ribonuclease P protein subunit p25 antibody
- RNase P protein subunit p25 antibody
Western blot - Anti-RPP25 antibody (ab126338)
Lane 2 : Anti-RPP25 antibody (ab126338)
Lane 1 : Marker
Lane 2 : SK-MEL-30 cell lysate
Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPP25 antibody (ab126338)
ab126338, at a dilution of 1/50, staining RPP25 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-RPP25 antibody (ab126338)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab126338.
Fixation/Permeabilization: PFA/Triton X-100
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"