Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 21 kDa.|
FunctionComponent of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA.
Sequence similaritiesBelongs to the histone-like Alba family.
- Information by UniProt
- FLJ20374 antibody
- Ribonuclease P protein subunit p25 antibody
- RNase P protein subunit p25 antibody
Lane 2 : Anti-RPP25 antibody (ab126338)
Lane 1 : Marker
Lane 2 : SK-MEL-30 cell lysate
Predicted band size: 21 kDa
ab126338, at a dilution of 1/50, staining RPP25 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli using ab126338.
Fixation/Permeabilization: PFA/Triton X-100
ab126338 has not yet been referenced specifically in any publications.