Overview

  • Product name

  • Description

    Rabbit polyclonal to RPP29
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with RPP29

  • Tested applications

    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human RPP29.

  • Positive control

    • HepG2 cell lysate
  • General notes

     This product was previously labelled as POP4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

Images

  • Lane 2 : Anti-RPP29 antibody (ab50948) at 1.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 25 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • ab50948 at 4&microg/ml staining RPP29 in paraffin-embedded sections of human kidney tissue. Human alveolar cells are positively labelled (indicated with arrows). Magnification: 400x.

References

ab50948 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Specification
Brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 29 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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