Anti-RPP30 antibody [2931D5a] (ab53744)

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Overview

  • Product name

    Anti-RPP30 antibody [2931D5a]

  • Description

    Mouse monoclonal [2931D5a] to RPP30

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Dot blotmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment (Human)

  • Positive control

    • HeLa whole cell lysate NIH3T3 whole cell lysate

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.

  • Clonality

    Monoclonal

  • Clone number

    2931D5a

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Dot blot Use at an assay dependent dilution.

Target

Images

  • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    anti Mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    anti Mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

References

ab53744 has not yet been referenced specifically in any publications.

Publishing research using ab53744? Please let us know so that we can cite the reference in this datasheet.

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