Overview

  • Product name
    Anti-RPP30 antibody [2931D5a]
    See all RPP30 primary antibodies
  • Description
    Mouse monoclonal [2931D5a] to RPP30
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa whole cell lysate NIH3T3 whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • Clonality
    Monoclonal
  • Clone number
    2931D5a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Dot blot Use at an assay dependent dilution.

Target

Images

  • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    anti Mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
    Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    anti Mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

References

ab53744 has not yet been referenced specifically in any publications.

Publishing research using ab53744? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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