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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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Anti-RPP30 antibody [2931D5a] (ab53744)

  • Datasheet
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Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
  • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)

Key features and details

  • Mouse monoclonal [2931D5a] to RPP30
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human RPP30 protein (ab102557)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-RPP30 antibody [2931D5a]
  • Description

    Mouse monoclonal [2931D5a] to RPP30
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment (Human)

  • Positive control

    • HeLa whole cell lysate NIH3T3 whole cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    2931D5a
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human RPP30 protein (ab102557)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
Application Abreviews Notes
WB
1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Notes
WB
1/100. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • Target information above from: UniProt accession P78346 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10556 Human
    • Entrez Gene: 54364 Mouse
    • Omim: 606115 Human
    • SwissProt: P78346 Human
    • SwissProt: O88796 Mouse
    • Unigene: 139120 Human
    • Unigene: 277891 Mouse
    • Alternative names

      • FLJ38491 antibody
      • Ribonuclease P protein subunit p30 antibody
      • RNase P subunit 2 antibody
      • RNaseP protein p30 antibody
      • RNASEP2 antibody
      • RP11-320F15.1 antibody
      • RPP30 antibody
      • RPP30_HUMAN antibody
      • TSG15 antibody
      see all

    Images

    • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
      Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
      Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution + HeLa whole cell lysate at 25 µg

      Secondary
      anti Mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 30 kDa
      Observed band size: 30 kDa

    • Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
      Western blot - Anti-RPP30 antibody [2931D5a] (ab53744)
      Anti-RPP30 antibody [2931D5a] (ab53744) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

      Secondary
      anti Mouse IgG at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 30 kDa
      Observed band size: 30 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab53744? Please let us know so that we can cite the reference in this datasheet.

    ab53744 has not yet been referenced specifically in any publications.

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