Product nameAnti-RPP40 antibody
See all RPP40 primary antibodies
DescriptionRabbit polyclonal to RPP40
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human RPP40 (internal sequence). The exact sequence is proprietary.
Database link: O75818
- WB: HEK-293T, HeLa, A431 and HepG2 whole cell extracts. ICC/IF: SK-N-SH cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- EC 184.108.40.206 antibody
- Ribonuclease P protein subunit p40 antibody
- RNase P subunit 1 antibody
All lanes : Anti-RPP40 antibody (ab228728) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
10% SDS-PAGE gel.
SK-N-SH (human neuroblastoma cell line) cells stained for RPP40 (green) using ab228728 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
ab228728 has not yet been referenced specifically in any publications.