Key features and details
- Rabbit polyclonal to RPP40
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-RPP40 antibody
See all RPP40 primary antibodies
DescriptionRabbit polyclonal to RPP40
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Rat RPP40 aa 91-259. N-terminal tags. Expressed in E. coli.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFMDLSLNLDSKKYRR ISWSFKEKKPLKFDFLLAWHHTGTEESTMMSYFSKYQIREHQPKVALSTV RDLQCPVLQSSSLAGEPEEACNALEFFDWLGAVFCNADLNNEPHNFISTY CCPQPNTVAAQACLCTITGFVLPEKILVLLEQLCHYFDEPKLAPWVTLTV QGFAD
Database link: Q5BK64-2
- IHC-P: Rat spleen tissue. WB: Recombinant rat RPP40 protein. Rat stomach and spleen lysate. HeLa cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml.|
FunctionComponent of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- EC 184.108.40.206 antibody
- Ribonuclease P protein subunit p40 antibody
- RNase P subunit 1 antibody
Formalin-fixed, paraffin-embedded rat spleen tissue stained for RPP40 with ab231621 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-RPP40 antibody (ab231621) at 2 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Anti-RPP40 antibody (ab231621) at 5 µg/ml + Rat spleen lysate
Anti-RPP40 antibody (ab231621) at 2 µg/ml + Rat stomach lysate
Anti-RPP40 antibody (ab231621) at 2 µg/ml + Recombinant rat RPP40 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231621 has not yet been referenced specifically in any publications.