Anti-RPRD2 antibody (ab10363)

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Western blot - Anti-RPRD2 antibody (ab10363)

    Key features and details

    • Goat polyclonal to RPRD2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-RPRD2 antibody
      See all RPRD2 primary antibodies

    • Description

      Goat polyclonal to RPRD2

    • Host species

      Goat

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

    • Immunogen

      Synthetic peptide corresponding to Human RPRD2. (LocusLink ID 23248)

    • Positive control

      • Whole cell lysate from HeLa cells.

    • General notes

       This product was previously labelled as KIAA0460

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10363 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Detects a band of approximately 220 kDa (predicted molecular weight: 160 kDa).
    Notes
    WB
    1/1000 - 1/5000. Detects a band of approximately 220 kDa (predicted molecular weight: 160 kDa).

    Target

    • Relevance

      This is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.

    • Cellular localization

      Nuclear

    • Database links

      • Alternative names

        • FLJ 32145 antibody
        • HSPC 099 antibody
        • HSPC099 antibody
        • Hypothetical Protein DKFZp686H22247 antibody
        • Hypothetical protein KIAA0460 antibody
        • KIAA 0460 antibody
        • Regulation of nuclear pre mRNA domain containing protein 2 antibody
        • Regulation of nuclear pre-mRNA domain containing 2 antibody
        • RPRD 2 antibody
        • RPRD2 antibody
        see all

      Images

      • Western blot - Anti-RPRD2 antibody (ab10363)
        Western blot - Anti-RPRD2 antibody (ab10363)

        ab10363 detection of human RPRD2 by Western blot. Samples: Whole cell lysate (WCL; amounts indicated) from HeLa cells. Antibody: ab10363 used at 1 mg/ml. Detection: Chemiluminescence with 3 minute exposure.

      References (1)

      Publishing research using ab10363? Please let us know so that we can cite the reference in this datasheet.

      ab10363 has been referenced in 1 publication.

      • Lv XJ  et al. RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol Rep : (2016). WB ; Mouse . PubMed: 26782095

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Question

      I am very interested in this antibody. However, I have some questions that I could not find in you website. 1: Could you tell me the epitope of the antibody? 2: the protein of KIAA0460 is about 1440 amino acid, however the protein detected by this antibody is about 220kDa, How to explain? 3: Most important, how do you know this protein involved in DNA repair and DNA damage? I did not find any paper show this.

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      Answer

      Thank you for your interest. The precise peptides used in the production of the antibodies ab10363 and ab10373 to KIAA0460 are proprietary. The antibody ab10363 is to a peptide that is encoded within the 5’ end of exon 11. The antibody ab10373 is to a peptide that is encoded within the 3’ end of exon 11. According to NCBI’s evidence viewer: (http://www.ncbi.nlm.nih.gov/sutils/evv.cgi?contig=NT_032962.5&gene=KIAA0460&lid=23248), Exon 11 encodes residues 529 to 1454 of BAA32305.2. The apparent MW on SDS-PAGE gels under the conditions in which the antibodies to KIAA0460 were tested is slightly larger than 220 kDa. Supporting evidence for this are: 1) both antibodies recognize a protein of the same Mr despite being generated to peptides that have nothing in common other than both are unique to KIAA0460 and 2) the antibodies were generated after KIAA0460 was identified as a protein that co-immunoprecipited with proteins that are established to be involved in repair of DNA damage. The gel slice, from which KIAA0460 was identified in this complex, was taken from the 220 to 240 Mr region of the gel. Owing to contractual obligations, the complex from which KIAA0460 was identified cannot be divulged prior to publication of these data.

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