Overview

  • Product name

  • Description

    Goat polyclonal to RPRD2
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human RPRD2 aa 1000-1050.
    Sequence:

    FKNMLKNASRKPSDDKHFGQA

  • Positive control

    • HEK293 cells
  • General notes

     This product was previously labelled as KIAA0460

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to RPRD2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 155 kDa).
IP Use at an assay dependent dilution.

Target

  • Relevance

    This is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • FLJ 32145 antibody
    • HSPC 099 antibody
    • HSPC099 antibody
    • Hypothetical Protein DKFZp686H22247 antibody
    • Hypothetical protein KIAA0460 antibody
    • KIAA 0460 antibody
    • Regulation of nuclear pre mRNA domain containing protein 2 antibody
    • Regulation of nuclear pre-mRNA domain containing 2 antibody
    • RPRD 2 antibody
    • RPRD2 antibody
    see all

Images

  • Lanes 1-2 : Anti-RPRD2 antibody (ab17996) at 1 µg/ml
    Lanes 3-4 : Anti-RPRD2 antibody (ab17996) at 0.4 µg/ml

    Lanes 1 & 3 : Whole cell lysate from HEK239 cells at 15 µg
    Lanes 2 & 4 : Whole cell lysate from HEK239 cells at 50 µg

    Predicted band size: 155 kDa
    Observed band size: 225 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Detection of Human RPRD2 by Western Blot.

    Samples: Whole cell lysate (15 and 50 µg) from HEK293 cells.

    Antibody: Affinity purified goat anti-RPRD2 antibody used at 1 and 0.4 mg/ml.

    Detection: Chemiluminescence with an exposure time of 1 minute.

References

ab17996 has not yet been referenced specifically in any publications.

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