Key features and details
- Goat polyclonal to RPRD2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPRD2 antibody
See all RPRD2 primary antibodies
DescriptionGoat polyclonal to RPRD2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HEK293 cells
This product was previously labelled as KIAA0460
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to RPRD2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab17996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 155 kDa).|
|IP||Use at an assay dependent dilution.|
RelevanceThis is a hypothetical protein fragment believed to be involved in DNA repair. KIAA0460 contains 1 CID domain.
- FLJ 32145 antibody
- HSPC 099 antibody
- HSPC099 antibody
Lanes 1-2 : Anti-RPRD2 antibody (ab17996) at 1 µg/ml
Lanes 3-4 : Anti-RPRD2 antibody (ab17996) at 0.4 µg/ml
Lanes 1 & 3 : Whole cell lysate from HEK239 cells at 15 µg
Lanes 2 & 4 : Whole cell lysate from HEK239 cells at 50 µg
Predicted band size: 155 kDa
Observed band size: 225 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Detection of Human RPRD2 by Western Blot.
Samples: Whole cell lysate (15 and 50 µg) from HEK293 cells.
Antibody: Affinity purified goat anti-RPRD2 antibody used at 1 and 0.4 mg/ml.
Detection: Chemiluminescence with an exposure time of 1 minute.
ab17996 has not yet been referenced specifically in any publications.