Product nameAnti-RPS12 antibody
See all RPS12 primary antibodies
DescriptionRabbit polyclonal to RPS12
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
Synthetic peptide within Human RPS12 aa 1-50. The exact sequence is proprietary.
Database link: P25398
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226358 was affinity purified using an epitope specific to RPS12 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/10000 - 1/25000. Predicted molecular weight: 15 kDa.|
Sequence similaritiesBelongs to the ribosomal protein S12e family.
- Information by UniProt
- 40S ribosomal protein S12 antibody
- ribosomal protein S12 antibody
- rps12 antibody
All lanes : Anti-RPS12 antibody (ab226358) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 15 kDa
Exposure time: 3 seconds
Cell lysates were prepared using NETN lysis buffer.
RPS12 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226358 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226358 at 0.4 µg/ml.
Lane 1: ab226358 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab226358 has not yet been referenced specifically in any publications.