Product nameAnti-RPS12 antibody [EPR10963]
See all RPS12 primary antibodies
DescriptionRabbit monoclonal [EPR10963] to RPS12
Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS12 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P25398
- HeLa, MCF-7, HepG2, Raji, C6, Raw264.7 and NIH-3T3 cell lysates; MCF7 cells; Hela cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- MCF7 whole cell lysate (ab3871)
- NIH 3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- HepG2 whole cell lysate (ab7900)
Our Abpromise guarantee covers the use of ab175219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Predicted molecular weight: 15 kDa.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
Sequence similaritiesBelongs to the ribosomal protein S12e family.
- Information by UniProt
- 40S ribosomal protein S12 antibody
- ribosomal protein S12 antibody
- rps12 antibody
All lanes : Anti-RPS12 antibody [EPR10963] (ab175219) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Raw 264.7 cell lysate
Lane 7 : NIH-3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 15 kDa
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).