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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS12 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P25398
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab175219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Predicted molecular weight: 15 kDa.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).
ab175219 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"