Overview

  • Product name

    Anti-RPS12 antibody [EPR10963]
    See all RPS12 primary antibodies
  • Description

    Rabbit monoclonal [EPR10963] to RPS12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IP, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS12 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P25398

  • Positive control

    • HeLa, MCF-7, HepG2, Raji, C6, Raw264.7 and NIH-3T3 cell lysates; MCF7 cells; Hela cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10963
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/5000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    Images

    • All lanes : Anti-RPS12 antibody [EPR10963] (ab175219) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Raji cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Raw 264.7 cell lysate
      Lane 7 : NIH-3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 15 kDa

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.

    • Flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red)  compared to a rabbit IgG negative control (green).

    • Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).

    References

    This product has been referenced in:

    • Luo Q  et al. mTORC1 Negatively Regulates the Replication of Classical Swine Fever Virus Through Autophagy and IRES-Dependent Translation. iScience 3:87-101 (2018). Read more (PubMed: 30428332) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175219.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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