Recombinant Anti-RPS12 antibody [EPR10964(B)] - BSA and Azide free (ab249439)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10964(B)] to RPS12 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RPS12 antibody [EPR10964(B)] - BSA and Azide free
See all RPS12 primary antibodies -
Description
Rabbit monoclonal [EPR10964(B)] to RPS12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249439 is the carrier-free version of ab167428.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10964(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 15 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the ribosomal protein S12e family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6206 Human
- Entrez Gene: 20042 Mouse
- Entrez Gene: 65139 Rat
- Omim: 603660 Human
- SwissProt: P25398 Human
- SwissProt: P63323 Mouse
- SwissProt: P63324 Rat
- Unigene: 546289 Human
see all -
Alternative names
- 40S ribosomal protein S12 antibody
- ribosomal protein S12 antibody
- rps12 antibody
see all
Images
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All lanes : Anti-RPS12 antibody [EPR10964(B)] (ab167428) at 1/1000 dilution
Lane 1 : Rat C6 cell lysate
Lane 2 : Mouse RAW 264.7 cell lysate
Lane 3 : Mouse NIH 3T3 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDaThis data was developed using ab167428, the same antibody clone in a different buffer formulation.
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This data was developed using ab167428, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling RPS12 using ab167428 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab167428, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling RPS12 using ab167428 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249439 has not yet been referenced specifically in any publications.