Overview

  • Product name
  • Description
    Rabbit polyclonal to RPS13
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Chinese hamster
  • Immunogen

    Synthetic peptide within Human RPS13 aa 89-118 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P62277

  • Positive control
    • NCI-H460 cells and lysates. Human breast carcinoma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 17 kDa.

Target

Images

  • Anti-RPS13 antibody (ab175107) at 1/1000 dilution + NCI-H460 cell lysate at 35 µg

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human breast carcinoma labeling RPS13 with ab175107 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) labeling RPS13 with ab175107 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

References

This product has been referenced in:
  • Wang Y  et al. Long noncoding RNA MRUL promotes ABCB1 expression in multidrug-resistant gastric cancer cell sublines. Mol Cell Biol 34:3182-93 (2014). Read more (PubMed: 24958102) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175107.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up