Key features and details
- Rabbit polyclonal to RPS14
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPS14 antibody
See all RPS14 primary antibodies
DescriptionRabbit polyclonal to RPS14
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- IHC-P: Human skin, colon, cerebral cortex and fallopian tube tissue. WB: RT4 cell lysate. Mouse cerebral cortex tissue lysate. ICC/IF: U-2 OS cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the ribosomal protein S11P family.
- Information by UniProt
- 40S ribosomal protein S14 antibody
- emetine resistance antibody
- EMTB antibody
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RPS14 using ab246916 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human skin tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human fallopian tube tissue stained for RPS14 using ab246916 at 1/50 dilution in immunohistochemical analysis.
Anti-RPS14 antibody (ab246916) at 0.4 µg/ml + Mouse cerebral cortex tissue lysate
Anti-RPS14 antibody (ab246916) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246916 has not yet been referenced specifically in any publications.