Recombinant Anti-RPS15 antibody [EPR11104] (ab157193)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11104] to RPS15
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-RPS15 antibody [EPR11104]
See all RPS15 primary antibodies -
Description
Rabbit monoclonal [EPR11104] to RPS15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide, corresponding to residues in Human RPS15 (UniProt: P62841).
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Positive control
- A549, HepG2, U-87 MG and MCF7 cell lysates; MCF7 and U-87 MG cells; Human brain tissue and Human esophageal carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11104 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Sequence similarities
Belongs to the ribosomal protein S19P family. - Information by UniProt
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Database links
- Entrez Gene: 6209 Human
- Entrez Gene: 20054 Mouse
- Entrez Gene: 29285 Rat
- Omim: 180535 Human
- SwissProt: P62841 Human
- SwissProt: P62843 Mouse
- SwissProt: P62845 Rat
- Unigene: 406683 Human
see all -
Alternative names
- 40S ribosomal protein S15 antibody
- Homolog of rat insulinoma antibody
- Insulinoma protein antibody
see all
Images
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All lanes : Anti-RPS15 antibody [EPR11104] (ab157193) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPST2 with ab157193 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labeling TPST2 with ab157193 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow cytometic analysis of permeabilized U-87 MG cells labeling TPST2 with ab157193 at 1/100 (red) or a rabbit IgG (negative) (green).
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Immunofluorescent analysis of MCF7 cells labeling TPST2 with ab157193 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab157193 has been referenced in 2 publications.
- Simbriger K et al. Monitoring translation in synaptic fractions using a ribosome profiling strategy. J Neurosci Methods 329:108456 (2020). PubMed: 31610213
- Cammarata-Mouchtouris A et al. Hyd ubiquitinates the NF-?B co-factor Akirin to operate an effective immune response in Drosophila. PLoS Pathog 16:e1008458 (2020). PubMed: 32339205