RecombinantRabMAb

Recombinant Anti-RPS15 antibody [EPR11104] (ab157193)

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Western blot - Anti-RPS15 antibody [EPR11104] (ab157193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
  • Flow Cytometry - Anti-RPS15 antibody [EPR11104] (ab157193)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11104] (ab157193)
  • Anti-RPS15 antibody [EPR11104] (ab157193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11104] to RPS15
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-RPS15 antibody [EPR11104]
    See all RPS15 primary antibodies

  • Description

    Rabbit monoclonal [EPR11104] to RPS15

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide, corresponding to residues in Human RPS15 (UniProt: P62841).

  • Positive control

    • A549, HepG2, U-87 MG and MCF7 cell lysates; MCF7 and U-87 MG cells; Human brain tissue and Human esophageal carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11104

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-RPS15 antibody [EPR11104] (ab157193)
      Western blot - Anti-RPS15 antibody [EPR11104] (ab157193)
      All lanes : Anti-RPS15 antibody [EPR11104] (ab157193) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : U-87 MG cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPST2 with ab157193 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)

      Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labeling TPST2 with ab157193 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-RPS15 antibody [EPR11104] (ab157193)
      Flow Cytometry - Anti-RPS15 antibody [EPR11104] (ab157193)
      Flow cytometic analysis of permeabilized U-87 MG cells labeling TPST2 with ab157193 at 1/100 (red) or a rabbit IgG (negative) (green).
    • Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunofluorescent analysis of MCF7 cells labeling TPST2 with ab157193 at 1/250 dilution.
    • Anti-RPS15 antibody [EPR11104] (ab157193)
      Anti-RPS15 antibody [EPR11104] (ab157193)

    References (2)

    Publishing research using ab157193? Please let us know so that we can cite the reference in this datasheet.

    ab157193 has been referenced in 2 publications.

    • Simbriger K  et al. Monitoring translation in synaptic fractions using a ribosome profiling strategy. J Neurosci Methods 329:108456 (2020). PubMed: 31610213
    • Cammarata-Mouchtouris A  et al. Hyd ubiquitinates the NF-?B co-factor Akirin to operate an effective immune response in Drosophila. PLoS Pathog 16:e1008458 (2020). PubMed: 32339205

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