Recombinant
RabMAb

Recombinant Anti-RPS15 antibody [EPR11104] (ab157193)

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Overview

  • Product name

    Anti-RPS15 antibody [EPR11104]
    See all RPS15 primary antibodies

  • Description

    Rabbit monoclonal [EPR11104] to RPS15

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to residues in Human RPS15 (UniProt: P62841).

  • Positive control

    • A549, HepG2, U-87 MG and MCF7 cell lysates; MCF7 and U-87 MG cells; Human brain tissue and Human esophageal carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11104

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Western blot - Anti-RPS15 antibody [EPR11104] (ab157193)
      Western blot - Anti-RPS15 antibody [EPR11104] (ab157193)
      All lanes : Anti-RPS15 antibody [EPR11104] (ab157193) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : U-87 MG cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPST2 with ab157193 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)

      Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labeling TPST2 with ab157193 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-RPS15 antibody [EPR11104] (ab157193)
      Flow Cytometry - Anti-RPS15 antibody [EPR11104] (ab157193)
      Flow cytometic analysis of permeabilized U-87 MG cells labeling TPST2 with ab157193 at 1/100 (red) or a rabbit IgG (negative) (green).
    • Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11104] (ab157193)
      Immunofluorescent analysis of MCF7 cells labeling TPST2 with ab157193 at 1/250 dilution.

    References

    ab157193 has not yet been referenced specifically in any publications.

    Publishing research using ab157193? Please let us know so that we can cite the reference in this datasheet.

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