Name: Anti-RPS15 antibody [EPR11104]
Brand: RabMAb
SKU: ab157193

Product name

Anti-RPS15 antibody [EPR11104]
See all RPS15 primary antibodies
Description

Rabbit monoclonal [EPR11104] to RPS15
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide, corresponding to residues in Human RPS15 (UniProt: P62841).
Positive control
- A549, HepG2, U-87 MG and MCF7 cell lysates; MCF7 and U-87 MG cells; Human brain tissue and Human esophageal carcinoma tissue.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11104
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab157193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IP.

Sequence similarities

Belongs to the ribosomal protein S19P family.
Target information above from: UniProt accession P62841 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6209 Human
- Entrez Gene: 20054 Mouse
- Entrez Gene: 29285 Rat
- Omim: 180535 Human
- SwissProt: P62841 Human
- SwissProt: P62843 Mouse
- SwissProt: P62845 Rat
- Unigene: 406683 Human
- Unigene: 643 Mouse
- Unigene: 3391 Rat
see all
Alternative names
- 40S ribosomal protein S15 antibody
- Homolog of rat insulinoma antibody
- Insulinoma protein antibody
- Ribosomal protein S15 antibody
- RIG antibody
- RIG protein antibody
- RPS 15 antibody
- rps15 antibody
- RS15_HUMAN antibody
- S15 antibody
see all

Western blot - Anti-RPS15 antibody [EPR11104] (ab157193)

All lanes : Anti-RPS15 antibody [EPR11104] (ab157193) at 1/1000 dilution

Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U-87 MG cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPST2 with ab157193 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS15 antibody [EPR11104] (ab157193)

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labeling TPST2 with ab157193 at 1/100 dilution.
Flow Cytometry - Anti-RPS15 antibody [EPR11104] (ab157193)

Flow cytometic analysis of permeabilized U-87 MG cells labeling TPST2 with ab157193 at 1/100 (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11104] (ab157193)

Immunofluorescent analysis of MCF7 cells labeling TPST2 with ab157193 at 1/250 dilution.

ab157193 has not yet been referenced specifically in any publications.

Publishing research using ab157193? Please let us know so that we can cite the reference in this datasheet.

Anti-RPS15 antibody [EPR11104] (ab157193)

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