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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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RecombinantRabMAb

Recombinant Anti-RPS15 antibody [EPR11105] (ab168361)

  • Datasheet
  • SDS
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Western blot - Anti-RPS15 antibody [EPR11105] (ab168361)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11105] (ab168361)
  • Anti-RPS15 antibody [EPR11105] (ab168361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11105] to RPS15
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RPS15 antibody [EPR11105]
    See all RPS15 primary antibodies
  • Description

    Rabbit monoclonal [EPR11105] to RPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human RPS15.

  • Positive control

    • MCF7, HepG2, A549, and U-87 MG cell lysates; HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11105
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Alternative Versions

    • Anti-RPS15 antibody [EPR11105] - BSA and Azide free (ab249457)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab168361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Sequence similarities

      Belongs to the ribosomal protein S19P family.
    • Target information above from: UniProt accession P62841 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6209 Human
      • Entrez Gene: 20054 Mouse
      • Entrez Gene: 29285 Rat
      • Omim: 180535 Human
      • SwissProt: P62841 Human
      • SwissProt: P62843 Mouse
      • SwissProt: P62845 Rat
      • Unigene: 406683 Human
      • Unigene: 643 Mouse
      • Unigene: 3391 Rat
      see all
    • Alternative names

      • 40S ribosomal protein S15 antibody
      • Homolog of rat insulinoma antibody
      • Insulinoma protein antibody
      • Ribosomal protein S15 antibody
      • RIG antibody
      • RIG protein antibody
      • RPS 15 antibody
      • rps15 antibody
      • RS15_HUMAN antibody
      • S15 antibody
      see all

    Images

    • Western blot - Anti-RPS15 antibody [EPR11105] (ab168361)
      Western blot - Anti-RPS15 antibody [EPR11105] (ab168361)
      All lanes : Anti-RPS15 antibody [EPR11105] (ab168361) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 17 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11105] (ab168361)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS15 antibody [EPR11105] (ab168361)
      Immunofluorescent analysis of HepG2 cells labeling RPS15 with ab168361 at 1/100 dilution.
    • Anti-RPS15 antibody [EPR11105] (ab168361)
      Anti-RPS15 antibody [EPR11105] (ab168361)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab168361? Please let us know so that we can cite the reference in this datasheet.

    ab168361 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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