• Product name
  • Description
    Rabbit polyclonal to RPS16
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Chinese hamster
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A region within a synthetic peptide: MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK, corresponding to N terminal amino acids 1-50 of Human RPS16

  • Positive control
    • Jurkat cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab26159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS16 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • Database links
  • Alternative names
    • 40S ribosomal protein S16 antibody
    • Ribosomal protein S16 antibody
    • RPS 16 antibody
    • S16 antibody


  • Anti-RPS16 antibody (ab26159) at 1.25 µg/ml + Jurkat cell lysate

    Predicted band size: 16 kDa

  • Staining of human lung tissue with ab26159 (4-8 ug/ml). Positive labelled aleveolar cells are indicated with arrows. 400X magnification.
  • ICC/IF image of ab26159 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26159, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-RPS16 antibody (ab26159) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 16 kDa


This product has been referenced in:
  • Sbarrato T  et al. A ribosome-related signature in peripheral blood CLL B cells is linked to reduced survival following treatment. Cell Death Dis 7:e2249 (2016). Read more (PubMed: 27253413) »
  • Lyabin DN & Ovchinnikov LP Selective regulation of YB-1 mRNA translation by the mTOR signaling pathway is not mediated by 4E-binding protein. Sci Rep 6:22502 (2016). WB . Read more (PubMed: 26931209) »

See all 7 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihre Email.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Chinese Hamster Cell lysate - whole cell (CHO DG44)
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Sven Mathias

Verified customer

Submitted Aug 27 2012

Vielen Dank für Ihre Antwort.

Um Ihre Bestellung aufnehmen zu können, brauche ich noch die Steuernummer Ihres Instituts. Falls wir die Bestellung durchführen ohne die Steuernummer, müssen Sie leider englische Umsatzsteuer ...

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Vielen Dank für Ihre Emailund für Ihr Interesse an unseren Produkten.

Leider ist die Homologie zwischen Chinese Hamster nicht ideal. Wir gehen davon aus dass ein Antikörper kreuzreagiert, wenn 85% oder mehrHomologie vorliegt. B...

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Vielen Dank für Ihre Anfrage.

Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so...

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